2008年11月23号，我的论文"MFEprimer: Multiple factor evaluation of the specificity of PCR primers“被Bioinformatics接收，呵呵

MFEprimer: Multiple factor evaluation of the specificity of PCR primers

Wubin Qu ^1,2, Zhiyong Shen ¹, Dongsheng Zhao ³, Yi Yang ^2,* and Chenggang Zhang <sup>1,*

1</sup>Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Beijing 100850, China.
²College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, China.3Beijing Institute of Health Service and Medical Information, Beijing 100850, China.

*To whom correspondence should be addressed.

Abstract
Summary: We developed a program named MFEprimer for evaluating the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3' end of the primer, melting temperature, GC content, and number of binding sites between the primer and DNA templates. MFEprimer can help the user to select more suitable primers before running either standard or multiplex PCR reactions. The cDNA and genomic DNA databases of 10 widely used species, as well as user custom databases, were used as DNA templates for analyzing primers specificity. Furthermore, we maintained a Primer3Plus server with a modified Primer3Manager for one-stop primer design and specificity checking.
Availability: The web service of MFEprimer is freely available at http://biocompute.bmi.ac.cn/MFEprimer/. A stand-alone version of MFEprimer can be downloaded at http://biocompute.bmi.ac.cn/MFEprimer/download/. Primer3Plus on our server is located at http://biocompute.bmi.ac.cn/MFEprimer/primer3plus.cgi. All programs are licensed under the General Public License (Free Software Foundation, 1991).
Contact: CZ (zhangcg@bmi.ac.cn)


Citation: Wubin Qu, Zhiyong Shen, Dongsheng Zhao, Yi Yang, and Chenggang Zhang. (2009) MFEprimer: multiple factor evaluation of the specificity of PCR primers, Bioinformatics, 25, 276-278.